Overview

  • Product nameAnti-CtBP1 antibody
    See all CtBP1 primary antibodies
  • Description
    Rabbit polyclonal to CtBP1
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non phosphopeptide derived from human CtBP1 around the phosphorylation site of serine 422 (A-P-SP-P-G).

  • Positive control
    • Extracts from Jurkat cells. Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab79417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 49 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionInvolved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.
  • Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Post-translational
    modifications
    The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.
    ADP-ribosylated; when cells are exposed to brefeldin A.
    Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BARS antibody
    • brefeldin A- ribosylated substrate antibody
    • C terminal binding protein 1 antibody
    • C-terminal-binding protein 1 antibody
    • CTBP antibody
    • CtBP1 antibody
    • CTBP1_HUMAN antibody
    • MGC104684 antibody
    see all

Anti-CtBP1 antibody images

  • All lanes : Anti-CtBP1 antibody (ab79417) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • ab79417, at a 1/50 dilution, staining CtBP1 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left panel) or presence (right panel) of the immunizing peptide.
  • ab79417 staining CtBP1 in human glioblastoma cell line D54MG by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 0.5% BSA for 20 minutes at room temperature. The primary antibody was diluted 1/60 in 0.5% BSA/PBS and incubated with the sample for 16 hours at 4°C. The secondary antibody was TRITC-conjugated Goat anti-Rabbit polyclonal, diluted 1/300.

    See Abreview

  • ICC/IF image of ab79417 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79417, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CtBP1 antibody (ab79417)

ab79417 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (glioma cell line C6 (rat))
Specification glioma cell line C6 (rat)
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line D54MG)
Specification human glioblastoma cell line D54MG
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"