FunctionInvolved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.
Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
Post-translational modificationsThe level of phosphorylation appears to be regulated during the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation. ADP-ribosylated; when cells are exposed to brefeldin A. Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.
ab79417, at a 1/50 dilution, staining CtBP1 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left panel) or presence (right panel) of the immunizing peptide.
Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody (ab79417)This image is courtesy of an anonymous Abreview.
ab79417 staining CtBP1 in human glioblastoma cell line D54MG by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 0.5% BSA for 20 minutes at room temperature. The primary antibody was diluted 1/60 in 0.5% BSA/PBS and incubated with the sample for 16 hours at 4°C. The secondary antibody was TRITC-conjugated Goat anti-Rabbit polyclonal, diluted 1/300.
ICC/IF image of ab79417 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79417, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-CtBP1 antibody (ab79417)
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