Anti-CtBP1 (phospho S422) antibody (ab92577)

Overview

  • Product nameAnti-CtBP1 (phospho S422) antibody
    See all CtBP1 primary antibodies
  • Description
    Rabbit polyclonal to CtBP1 (phospho S422)
  • SpecificityDetects endogenous levels of CtBP1 only when phosphorylated at Serine 422.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human CtBP1 around the phosphorylation site of Serine 422 (A-P-SP-P-G).

  • Positive control
    • WB: lysate from Jurkat cells pre-treated with TNF (20ng/ml, 30mins); IHC-P: human brain tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab92577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: 1/5000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 49 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.
    • Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
    • Post-translational
      modifications
      The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.
      ADP-ribosylated; when cells are exposed to brefeldin A.
      Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BARS antibody
      • brefeldin A- ribosylated substrate antibody
      • C terminal binding protein 1 antibody
      • C-terminal-binding protein 1 antibody
      • CTBP antibody
      • CtBP1 antibody
      • CTBP1_HUMAN antibody
      • MGC104684 antibody
      see all

    Anti-CtBP1 (phospho S422) antibody images

    • All lanes : Anti-CtBP1 (phospho S422) antibody (ab92577) at 1/500 dilution

      Lane 1 : lysate from Jurkat cells treated with TNF (20ng/ml, 30mins)
      Lane 2 : lysate from Jurkat cells treated with TNF (20ng/ml, 30mins)
      Lane 3 : lysate from Jurkat cells treated with TNF (20ng/ml, 30mins) with immunizing phosphopeptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 49 kDa
    • Left panel: ab92577, at a 1/50 dilution, staining phosphorylated CtBP1 in formalin/ fixed, paraffin embedded Human tissue brain, using Immunohistochemistry.
      Right panel: same, after pre-incubation with the immunizing phosphopeptide.

    References for Anti-CtBP1 (phospho S422) antibody (ab92577)

    ab92577 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"