FunctionCorepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation. Isoform 2 probably acts as a scaffold for specialized synapses.
Tissue specificityUbiquitous. Highest levels in heart, skeletal muscle, and pancreas.
Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
Post-translational modificationsIsoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
All lanes : Anti-CTBP2 antibody (ab113265) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 49 kDa
Exposure time : 3 minutes
Immunoprecipitation - CTBP2 antibody (ab113265)
Lane 1: 1mg whole cell lysate from HeLa cells was immunoprecipitated with ab113265 at 6µg/mg lysate and 20% IP was loaded on gel. Lane 2: same, with a control IgG.
For WB ab113265 was used at 1µg/ml. Detection:
chemiluminescence with exposure times of 30 seconds.
References for Anti-CTBP2 antibody (ab113265)
has not yet been referenced specifically in any publications.
Publishing research using ab113265? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"