Overview

  • Product nameAnti-CTBP2 antibody
    See all CTBP2 primary antibodies
  • Description
    Mouse polyclonal to CTBP2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    CTBP2 (AAH37900, 1 a.a. ~ 513 a.a) full-length human protein.

  • Positive control
    • Transfected 293T cell lysate, human pancreas

Properties

Applications

Our Abpromise guarantee covers the use of ab67161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).

Target

  • FunctionCorepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
    Isoform 2 probably acts as a scaffold for specialized synapses.
  • Tissue specificityUbiquitous. Highest levels in heart, skeletal muscle, and pancreas.
  • Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Post-translational
    modifications
    Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
  • Cellular localizationNucleus. Cell junction > synapse.
  • Information by UniProt
  • Database links
  • Alternative names
    • C terminal binding protein 2 antibody
    • C-terminal-binding protein 2 antibody
    • CtBP2 antibody
    • CTBP2_HUMAN antibody
    • ribeye antibody
    see all

Anti-CTBP2 antibody images

  • Anti-CTBP2 antibody (ab67161) at 1/500 dilution + human pancreas at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 49 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CTBP2 antibody (ab67161) at 1/500 dilution

    Lane 1 : Transfected 293T cell lysate
    Lane 2 : non Transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 49 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-CTBP2 antibody (ab67161)

ab67161 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Mouse Tissue lysate - whole (brain, total, prefrontal cortex, hippocampus, embr)
Specification brain, total, prefrontal cortex, hippocampus, embr
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Evgenia Salta

Verified customer

Submitted Jul 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"