Overview

  • Product nameAnti-CTBP2 antibody
    See all CTBP2 primary antibodies
  • Description
    Rabbit polyclonal to CTBP2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Monkey, Zebrafish
  • Immunogen

    Recombinant full length protein, corresponding to a region within amino acids 737-927 of Human CTBP2 (NP_073713).

  • Positive control
    • Molt-4 whole cell lysate (WB) and HeLa cell (IF)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 106 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionCorepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
    Isoform 2 probably acts as a scaffold for specialized synapses.
  • Tissue specificityUbiquitous. Highest levels in heart, skeletal muscle, and pancreas.
  • Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Post-translational
    modifications
    Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
  • Cellular localizationNucleus. Cell junction > synapse.
  • Information by UniProt
  • Database links
  • Alternative names
    • C terminal binding protein 2 antibody
    • C-terminal-binding protein 2 antibody
    • CtBP2 antibody
    • CTBP2_HUMAN antibody
    • ribeye antibody
    see all

Anti-CTBP2 antibody images

  • Anti-CTBP2 antibody (ab96107) at 1/500 dilution + Molt-4 whole cell lysate at 30 µg

    Predicted band size : 106 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96107 at 1/100 dilution.
  • Anti-CTBP2 antibody (ab96107) at 1/200 dilution + zebrafish embryo tissue lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/4000 dilution

    Predicted band size : 106 kDa
    Observed band size : 55-60,85-90 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Evgenia Salta

    See Abreview

References for Anti-CTBP2 antibody (ab96107)

ab96107 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 50 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing (4°12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Evgenia Salta

Verified customer

Submitted Jul 17 2012

Application Western blot
Sample Zebrafish Tissue lysate - whole (Whole-mount, Larvae, 3 dpf)
Loading amount 40 µg
Specification Whole-mount, Larvae, 3 dpf
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Evgenia Salta

Verified customer

Submitted Jul 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"