Overview

  • Product name
  • Description
    Rabbit polyclonal to CTBP2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Monkey, Zebrafish
  • Immunogen

    Recombinant full length protein, corresponding to a region within amino acids 737-927 of Human CTBP2 (NP_073713).

  • Positive control
    • Molt-4 whole cell lysate (WB) and HeLa cell (IF)

Properties

Applications

Our Abpromise guarantee covers the use of ab96107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 106 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
    Isoform 2 probably acts as a scaffold for specialized synapses.
  • Tissue specificity
    Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.
  • Sequence similarities
    Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Post-translational
    modifications
    Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
  • Cellular localization
    Nucleus. Cell junction > synapse.
  • Information by UniProt
  • Database links
  • Alternative names
    • C terminal binding protein 2 antibody
    • C-terminal-binding protein 2 antibody
    • CtBP2 antibody
    • CTBP2_HUMAN antibody
    • ribeye antibody
    see all

Images

  • Anti-CTBP2 antibody (ab96107) at 1/500 dilution + Molt-4 whole cell lysate at 30 µg

    Predicted band size : 106 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96107 at 1/100 dilution.
  • Anti-CTBP2 antibody (ab96107) at 1/200 dilution + zebrafish embryo tissue lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/4000 dilution

    Predicted band size : 106 kDa
    Observed band size : 55-60,85-90 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Evgenia Salta

    See Abreview

References

ab96107 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
50 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (4°12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Evgenia Salta

Verified customer

Submitted Jul 17 2012

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Whole-mount, Larvae, 3 dpf)
Loading amount
40 µg
Specification
Whole-mount, Larvae, 3 dpf
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Evgenia Salta

Verified customer

Submitted Jul 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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