Synthetic peptide corresponding to a region between residues 650 and 700 of human CTCF (Peptide available as ab115042.)
Our Abpromise guarantee covers the use of ab70303 in the following tested applications.
|WB||1/2500 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|ChIP||Use at an assay dependent concentration.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|EMSA||Use at an assay dependent concentration. PubMed: 22210889|
Samples: Whole cell lysate (50 µg) from CT26.WT, 4T1, Renca, and TCMK-1 cells. ab70303 used at a dilution of 0.2 µg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.