Overview

  • Product nameAnti-CTCF antibody [mAbcam 37477]
    See all CTCF primary antibodies
  • Description
    Mouse monoclonal [mAbcam 37477] to CTCF
  • SpecificityThis antibody has previously been successfully used in western blot after immunoprecipitation from mouse NIH3T3 lysates. Please see Yao H et al. Genes Dev 24:2543-55 (2010) PMID: 20966046 for more details.
  • Tested applicationsSuitable for: IP, Flow Cyt, WB, EMSAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment derived from the N terminal end of Human CTCF.

  • Positive control
    • This antibody gave a positive signal in MCF7 whole cell lysate and HeLa whole cell lysate.
  • General notesCTCF is known to migrate by SDS-PAGE at 130 kDa (PMID: 9016583) We can conjugate this antibody to FITC for you (please see ab150242 for details).

Properties

Applications

Our Abpromise guarantee covers the use of ab37477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa).
EMSA Use at an assay dependent concentration. PubMed: 22210889

Target

  • FunctionChromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates to the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19.
  • Tissue specificityUbiquitous. Absent in primary spermatocytes.
  • Sequence similaritiesBelongs to the CTCF zinc-finger protein family.
    Contains 11 C2H2-type zinc fingers.
  • DomainThe 11 zinc fingers are highly conserved among vertebrates, exhibiting almost identical amino acid sequences. Different subsets or combination of individual zinc fingers gives the ability to CTCF to recognize multiple DNA target sites.
  • Post-translational
    modifications
    Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter.
  • Cellular localizationNucleus > nucleoplasm. Chromosome. Chromosome > centromere. May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • 11 zinc finger protein antibody
    • 11 zinc finger transcriptional repressor antibody
    • 11-zinc finger protein antibody
    • CCCTC binding factor (zinc finger protein) antibody
    • CCCTC binding factor antibody
    • CCCTC-binding factor antibody
    • Ctcf antibody
    • CTCF_HUMAN antibody
    • CTCFL paralog antibody
    • MRD21 antibody
    • Transcriptional repressor CTCF antibody
    see all

Anti-CTCF antibody [mAbcam 37477] images

  • All lanes : Anti-CTCF antibody [mAbcam 37477] (ab37477) at 5 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 83 kDa


    Exposure time : 90 seconds
  • All lanes : Anti-CTCF antibody [mAbcam 37477] (ab37477) at 2 µg/ml

    Lane 1 : ZR-75-1 cell extract
    Lane 2 : HCT-116 cell extract


    Predicted band size : 83 kDa

    This image is courtesy of Elena Klenova, University of Essex, UK

    ab37477 recognises a band at approximately 130 kDa corresponding to CTCF.
  • Overlay histogram showing HeLa cells stained with ab37477 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab37477, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-CTCF antibody [mAbcam 37477] (ab37477)

This product has been referenced in:
  • Huang K  et al. Ribosomal RNA gene transcription mediated by the master genome regulator protein CCCTC-binding factor (CTCF) is negatively regulated by the condensin complex. J Biol Chem 288:26067-77 (2013). WB . Read more (PubMed: 23884423) »
  • Golan-Mashiach M  et al. Identification of CTCF as a master regulator of the clustered protocadherin genes. Nucleic Acids Res : (2011). EMSA . Read more (PubMed: 22210889) »

See all 3 Publications for this product

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