Anti-CTDSP1 antibody - C-terminal (ab76099)

Overview

  • Product nameAnti-CTDSP1 antibody - C-terminal
    See all CTDSP1 primary antibodies
  • Description
    Rabbit polyclonal to CTDSP1 - C-terminal
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    KLH conjugated synthetic peptide selected from the C terminal region of human CTDSP1 protein.

  • Positive control
    • CEM tissue lysate. 293 cell lysates transiently transfected with the CTDSP1 gene. Human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab76099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 29 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • FunctionPreferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.
  • Tissue specificityExpression is restricted to non-neuronal tissues. Highest expression in skeletal muscle, spleen, lung and placenta.
  • Sequence similaritiesContains 1 FCP1 homology domain.
  • Cellular localizationNucleus. Colocalizes with RNA polymerase II.
  • Information by UniProt
  • Database links
  • Alternative names
    • Carboxy terminal domain RNA polymerase II polypeptide A small phosphatase 1 antibody
    • Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 antibody
    • CTD (carboxy terminal domain RNA polymerase II polypeptide A) small phosphatase 1 antibody
    • CTDS1_HUMAN antibody
    • CTDSP1 antibody
    • NIF3 antibody
    • NLI IF antibody
    • NLI interacting factor 3 antibody
    • NLI-IF antibody
    • NLI-interacting factor 3 antibody
    • NLIIF antibody
    • Nuclear LIM interactor interacting factor 3 antibody
    • Nuclear LIM interactor-interacting factor 3 antibody
    • SCP1 antibody
    • Small C-terminal domain phosphatase 1 antibody
    • Small CTD phosphatase 1 antibody
    see all

Anti-CTDSP1 antibody - C-terminal images

  • Anti-CTDSP1 antibody - C-terminal (ab76099) at 1/100 dilution + CEM tissue lysate at 12.5 µg

    Predicted band size : 29 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-CTDSP1 antibody - C-terminal (ab76099) at 1/100 dilution

    Lane 1 : Nontransfected 293 cell lysates
    Lane 2 : 293 cell lysates transiently transfected with the CTDSP1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.
  • ab76099, at 1/50 dilution, staining CTDSP1 in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue by Immunohistochemistry, followed by a peroxidase conjugated secondary antibody and DAB staining.

References for Anti-CTDSP1 antibody - C-terminal (ab76099)

ab76099 has not yet been referenced specifically in any publications.

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