Overview

  • Product name
  • Description
    Rabbit polyclonal to CTDSPL
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 48-97 (CCFRDYNVEA PPPSSPSVLP PLVEENGGLQ KPPAKYLLPE VTVLDYGKKC) of Human CTDSPL (NP_005799).

  • Positive control
    • DLD1 cell lysate; Human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab105068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (use citrate buffer pH 6.0)

Target

  • Function
    Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation (By similarity). Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.
  • Tissue specificity
    Expression is restricted to non-neuronal tissues.
  • Sequence similarities
    Contains 1 FCP1 homology domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C3orf8 antibody
    • Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3 antibody
    • CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like antibody
    • CTD small phosphatase-like protein antibody
    • CTDSL_HUMAN antibody
    • CTDSP-like antibody
    • Ctdspl antibody
    • hYA22 antibody
    • N-like protein antibody
    • NIF-like protein antibody
    • NLI-interacting factor 1 antibody
    • Nuclear LIM interactor-interacting factor 1 antibody
    • Protein YA22 antibody
    • RB protein serine phosphatase from chromosome 3 antibody
    • RBSP3 antibody
    • SCP3 antibody
    • Small C-terminal domain phosphatase 3 antibody
    • Small CTD phosphatase 3 antibody
    see all

Images

  • Anti-CTDSPL antibody (ab105068) at 1 µg/ml + DLD1 cell lysate at 10 µg

    Predicted band size : 31 kDa
    Gel concentration: 15%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CTDSPL with ab105068 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.

References

ab105068 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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