The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
Preliminary experiments gave an approx 20kDa band in human placenta lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 31.1kDa according to NP_NP_001008393.1 and of 29.9kDa according to NP_005799.2. The 20kDa band was successfully blocked by incubation with the immunizing peptide
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPreferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation (By similarity). Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.
Tissue specificityExpression is restricted to non-neuronal tissues.