Overview

  • Product nameAnti-CTGF antibody
    See all CTGF primary antibodies
  • Description
    Rabbit polyclonal to CTGF
  • SpecificityUeberham et al (Neuroscience, 2003)report "In human hippocampal protein extracts two distinct bands are detectable with a more intensive reactivity at about 37-38 kDa, representing the CTGF protein, and at about 42-44 kDa likely representing a glycosilated ... or a phosphorylated form of CTGF...".
  • Tested applicationsSuitable for: IP, ICC/IF, WB, IHC-P, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Mouse CTGF aa 223-348.
    Database link: P29268
    (Peptide available as ab7861)

  • Positive control
    • human hippocampal protein extracts (see Ueberham et al paper).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 16100286
ICC/IF 1/200.
WB 1/1000. Can be blocked with Mouse CTGF peptide (ab7861).

Block membrane in 4% BSA in Tris-buffered saline containing 0.05% Tween 20 (TBST). See Fu M et al.

IHC-P 1/400 - 1/800. Do not perform antigen retrieval.
IHC-Fr Use at an assay dependent concentration. See Ueberham et al.
IHC-FoFr 1/100.

Target

  • FunctionMajor connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
  • Tissue specificityExpressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
  • Sequence similaritiesBelongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCN 2 antibody
    • CCN family member 2 antibody
    • CCN2 antibody
    • Connective tissue growth factor antibody
    • Ctgf antibody
    • CTGF_HUMAN antibody
    • Hcs 24 antibody
    • Hcs24 antibody
    • Hypertrophic chondrocyte specific protein 24 antibody
    • Hypertrophic chondrocyte-specific gene product 24 antibody
    • Hypertrophic chondrocyte-specific protein 24 antibody
    • IBP-8 antibody
    • IGF-binding protein 8 antibody
    • IGFBP-8 antibody
    • IGFBP8 antibody
    • Insulin like Growth Factor Binding Protein 8 antibody
    • Insulin-like growth factor-binding protein 8 antibody
    • MGC102839 antibody
    • NOV 2 antibody
    • NOV2 antibody
    see all

Anti-CTGF antibody images

  • ab6992 staining GTGF (green) in Human vasculature tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with mathacarn and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/400) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody. Blue - DAPI Nuclei.

    See Abreview

  • All lanes : Anti-CTGF antibody (ab6992) at 1/1000 dilution

    Lane 1 : Extracellular matric within skeletal muscle from a mouse with fibrosis in the legs
    Lane 2 : Human skeletal muscle whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa Fluor® 488-conjugated goat anti-rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size : 36 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab6992 at a 1/200 dilution staining CTGF in mouse liver tissue sections by Immunohistochemistry (frozen sections) incubated at +4°C for 9 hours. Fixed in formaldehyde, permeabilized using 0.2% Triton X-100. Blocked using 2% BSA for 30 minutes at 20°C. Secondary used at 1/200 dilution polyclonal Goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green).

    See Abreview

  • ab6992 staining CTGF in Human 293FT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in PBS) for 2 hours at 25°C. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab6992 at a 1/100 dilution staining CTGF from Mouse Tissue sections (Skin) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 12 hours and then detected using a Labeled AP polymer. The right side of image is regrown wounded epidermis and shows significant staining for CTGF.

    See Abreview

  • ab6992 staining GTGF (green) in Mouse vasculature tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with mathacarn and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/400) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody. Blue - DAPI Nuclei.

    See Abreview

  • ab6692 staining CTGF in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 12 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

  • ab6992 staining CTGF in Sheep articular cartilage tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% neutral-buffered formalin and blocked with 0.25% serum-free protein block for 10 minutes at room temperature. Samples were incubated with primary antibody (1/400) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.

    See Abreview

  • Anti-CTGF antibody (ab6992) at 1/5000 dilution (in PBS-T for 9 hours at 4°C) + Mouse brain whole cell lysate at 50 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Observed band size : 36 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 25°C

    See Abreview

References for Anti-CTGF antibody (ab6992)

This product has been referenced in:
  • Zheng X  et al. Renal Sympathetic Denervation in Rats Ameliorates Cardiac Dysfunction and Fibrosis Post-Myocardial Infarction Involving MicroRNAs. Med Sci Monit 22:2751-60 (2016). IHC-P ; Rat . Read more (PubMed: 27490896) »
  • Li C  et al. RhoA determines lineage fate of mesenchymal stem cells by modulating CTGF-VEGF complex in extracellular matrix. Nat Commun 7:11455 (2016). WB . Read more (PubMed: 27126736) »

See all 67 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (iPSC derived nuerons)
Specification iPSC derived nuerons
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Feb 26 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 15% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Sample Human Tissue sections (saphenous vein)
Specification saphenous vein
Permeabilization Yes - tween20
Fixative Formaldehyde
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Submitted Mar 19 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (retina)
Specification retina
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Mar 12 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% Bis-Tris)
Sample Human Tissue lysate - whole (Skeletal Muscle)
Specification Skeletal Muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Submitted Mar 03 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (Vasculature)
Specification Vasculature
Permeabilization No
Fixative Methacarn
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Submitted Nov 25 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (Vasculature)
Specification Vasculature
Permeabilization No
Fixative Methacarn
Username

Abcam user community

Verified customer

Submitted Nov 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Protein-Block Serum Free (DakoCytomation) as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Antigen retrieval step None
Sample Sheep Tissue sections (Articular Cartilage)
Specification Articular Cartilage
Permeabilization No
Fixative 10% neutral-buffered formalin
Username

Abcam user community

Verified customer

Submitted Jun 20 2013

Thank you for contacting us.

This antibody is already tested in applications and species customer is interested in using, which means the product is guaranteed for use hence the product is not eligible for Abtrial discount.

I hope thi...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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1-10 of 56 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"