Anti-CTGF antibody (Biotin) (ab84244)

Overview

  • Product nameAnti-CTGF antibody (Biotin)
    See all CTGF primary antibodies
  • Description
    Rabbit polyclonal to CTGF (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CTGF aa 253-349.
    Database link: P29279

Properties

  • FormLyophilised:Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab84244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/0.25 - 1/1.

To detect by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hCTGF.

Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to CTGF (ab109606).

To detect by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ab109606 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hCTGF.

WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 38 kDa.

Target

  • FunctionMajor connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
  • Tissue specificityExpressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
  • Sequence similaritiesBelongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCN 2 antibody
    • CCN family member 2 antibody
    • CCN2 antibody
    • Connective tissue growth factor antibody
    • Ctgf antibody
    • CTGF_HUMAN antibody
    • Hcs 24 antibody
    • Hcs24 antibody
    • Hypertrophic chondrocyte specific protein 24 antibody
    • Hypertrophic chondrocyte-specific gene product 24 antibody
    • Hypertrophic chondrocyte-specific protein 24 antibody
    • IBP-8 antibody
    • IGF-binding protein 8 antibody
    • IGFBP-8 antibody
    • IGFBP8 antibody
    • Insulin like Growth Factor Binding Protein 8 antibody
    • Insulin-like growth factor-binding protein 8 antibody
    • MGC102839 antibody
    • NOV 2 antibody
    • NOV2 antibody
    see all

References for Anti-CTGF antibody (Biotin) (ab84244)

ab84244 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

I can confirm that ab50044 can be used as a control in sELISA involving ab84244 and ab109606.

Please be advised the conditions and dilutions has to be determined by end use.

I hope this infor...

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Thank you for your phone call. It was nice talking to you.
I am currently in touch with our lab regarding the positive control protein you requested. I will get back to you with more information soon.

Sorry about the delay!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"