Overview

  • Product nameAnti-CtIP antibody - ChIP Grade
    See all CtIP primary antibodies
  • Description
    Rabbit polyclonal to CtIP - ChIP Grade
  • Tested applicationsSuitable for: ChIP, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Pig, Chimpanzee, Baboon, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human CtIP (C terminal).
    Database link: Q99708

  • Positive control
    • MCF7 and HeLa whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution.
WB 1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 102 kDa).
IP Use at 2 µg/mg of lysate.

Target

  • FunctionEndonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations.
  • Involvement in diseaseSeckel syndrome 2
    Jawad syndrome
    Genetic variability in RBBP8 is noted as a factor in BRCA1-associated breast cancer risk (PubMed:21799032). Exhibits sensitivity to tamoxifen in certain breast cancer cell lines (PubMed:18171986).
  • Sequence similaritiesBelongs to the COM1/SAE2/CtIP family.
  • DomainThe PXDLS motif binds to a cleft in CtBP proteins.
    The damage-recruitment motif is required for DNA binding and translocation to sites of DNA damage.
  • Post-translational
    modifications
    Acetylated. Deacetylation by SIRT6 upon DNA damage promotes DNA end resection.
    Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control.
    Ubiquitinated (PubMed:14654780, PubMed:16818604). Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteosomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control (PubMed:16818604). Ubiquitinated by RNF138 at its N-terminus (PubMed:26502057).
  • Cellular localizationNucleus. Chromosome. Associates with sites of DNA damage in S/G2 phase (PubMed:10764811). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604).
  • Information by UniProt
  • Database links
  • Alternative names
    • COM1 antibody
    • COM1_HUMAN antibody
    • CtBP interacting protein antibody
    • CtBP-interacting protein antibody
    • CtIP antibody
    • DNA endonuclease RBBP8 antibody
    • JWDS antibody
    • RBBP-8 antibody
    • RBBP8 antibody
    • Retinoblastoma-binding protein 8 antibody
    • Retinoblastoma-interacting protein and myosin-like antibody
    • Rim antibody
    • SAE2 antibody
    • SCKL2 antibody
    • Sporulation in the absence of SPO11 protein 2 homolog antibody
    see all

Anti-CtIP antibody - ChIP Grade images

  • CtIP was immunoprecipitated from 293T cells using NETN lysis buffer with ab70163 at 2ug/mg of lysate. 

    Chemiluminescence with an exposure time: 3 minutes

  • All lanes : Anti-CtIP antibody - ChIP Grade (ab70163) at 1 µg/ml

    Lane 1 : MCF7 whole cell lysate at 50 µg
    Lane 2 : MCF7 whole cell lysate at 15 µg
    Lane 3 : MCF7 whole cell lysate at 5 µg
    Lane 4 : HeLa whole cell lysate at 50 µg


    Predicted band size : 102 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

References for Anti-CtIP antibody - ChIP Grade (ab70163)

This product has been referenced in:
  • Silva BA  et al. DNA damage to a single chromosome end delays anaphase onset. J Biol Chem 289:22771-84 (2014). ICC/IF . Read more (PubMed: 24982423) »
  • Xu J  et al. APE1 is dispensable for S-region cleavage but required for its repair in class switch recombination. Proc Natl Acad Sci U S A 111:17242-7 (2014). Read more (PubMed: 25404348) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification Hela
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 01 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Saccharomyces cerevisiae Cell lysate - whole cell (exponentially growing yeast cells)
Loading amount 1e+007 cells
Specification exponentially growing yeast cells
Gel Running Conditions Reduced Denaturing (gel 10% SDS page)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Ilaria Guerini

Verified customer

Submitted Aug 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"