Recombinant
RabMAb

Anti-CtIP antibody [EPNCIR160] (ab155988)

Overview

  • Product name
    Anti-CtIP antibody [EPNCIR160]
    See all CtIP primary antibodies
  • Description
    Rabbit monoclonal [EPNCIR160] to CtIP
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CtIP.
    Database link: Q99708

  • Positive control
    • Jurkat and MCF-7 cell lysates; Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Andre Nussenzweig.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab155988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 101 kDa.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations.
    • Involvement in disease
      Seckel syndrome 2
      Jawad syndrome
      Genetic variability in RBBP8 is noted as a factor in BRCA1-associated breast cancer risk (PubMed:21799032). Exhibits sensitivity to tamoxifen in certain breast cancer cell lines (PubMed:18171986).
    • Sequence similarities
      Belongs to the COM1/SAE2/CtIP family.
    • Domain
      The PXDLS motif binds to a cleft in CtBP proteins.
      The damage-recruitment motif is required for DNA binding and translocation to sites of DNA damage.
    • Post-translational
      modifications
      Acetylated. Deacetylation by SIRT6 upon DNA damage promotes DNA end resection.
      Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control.
      Ubiquitinated (PubMed:14654780, PubMed:16818604). Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteosomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control (PubMed:16818604). Ubiquitinated by RNF138 at its N-terminus (PubMed:26502057).
    • Cellular localization
      Nucleus. Chromosome. Associates with sites of DNA damage in S/G2 phase (PubMed:10764811). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604).
    • Information by UniProt
    • Database links
    • Alternative names
      • COM1 antibody
      • COM1_HUMAN antibody
      • CtBP interacting protein antibody
      • CtBP-interacting protein antibody
      • CtIP antibody
      • DNA endonuclease RBBP8 antibody
      • JWDS antibody
      • RB binding protein 8 endonuclease antibody
      • RBBP-8 antibody
      • RBBP8 antibody
      • Retinoblastoma-binding protein 8 antibody
      • Retinoblastoma-interacting protein and myosin-like antibody
      • Rim antibody
      • SAE2 antibody
      • SCKL2 antibody
      • Sporulation in the absence of SPO11 protein 2 homolog antibody
      see all

    Images

    • All lanes : Anti-CtIP antibody [EPNCIR160] (ab155988) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : MCF-7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 101 kDa
    • Flow Cytometric analysis of permeabilized Jurkat cells labeling CtIP with ab155988 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

    References

    ab155988 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 01 2016

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Human Cell lysate - whole cell (Hela cells)
    Specification
    Hela cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Remi Buisson

    Verified customer

    Submitted Oct 30 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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