Anti-CtIP (phospho S738) antibody [EP18893] (ab195291)

Overview

  • Product name
    Anti-CtIP (phospho S738) antibody [EP18893]
    See all CtIP primary antibodies
  • Description
    Rabbit monoclonal [EP18893] to CtIP (phospho S738)
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide within Human CtIP aa 700-800 (phospho S738). The exact sequence is proprietary. (Co-immunization performed with both peptides, clone obtained by screening with peptide 1).
    Database link: Q99708

    Synthetic peptide within Human CtIP aa 700-800 (phospho S738). The exact sequence is proprietary. (Co-immunization performed with both peptides, clone obtained by screening with peptide 1).
    Database link: Q99708

  • Positive control
    • WB: HEK-293 untreated, and treated with 60 J/m² UV light, then cultivated in RPMI 1640 media containing 10% FBS for 2 hours, whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab195291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
Dot blot 1/1000.

Target

  • Function
    Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations.
  • Involvement in disease
    Seckel syndrome 2
    Jawad syndrome
    Genetic variability in RBBP8 is noted as a factor in BRCA1-associated breast cancer risk (PubMed:21799032). Exhibits sensitivity to tamoxifen in certain breast cancer cell lines (PubMed:18171986).
  • Sequence similarities
    Belongs to the COM1/SAE2/CtIP family.
  • Domain
    The PXDLS motif binds to a cleft in CtBP proteins.
    The damage-recruitment motif is required for DNA binding and translocation to sites of DNA damage.
  • Post-translational
    modifications
    Acetylated. Deacetylation by SIRT6 upon DNA damage promotes DNA end resection.
    Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control.
    Ubiquitinated (PubMed:14654780, PubMed:16818604). Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteosomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control (PubMed:16818604). Ubiquitinated by RNF138 at its N-terminus (PubMed:26502057).
  • Cellular localization
    Nucleus. Chromosome. Associates with sites of DNA damage in S/G2 phase (PubMed:10764811). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604).
  • Information by UniProt
  • Database links
  • Alternative names
    • COM1 antibody
    • COM1_HUMAN antibody
    • CtBP interacting protein antibody
    • CtBP-interacting protein antibody
    • CtIP antibody
    • DNA endonuclease RBBP8 antibody
    • JWDS antibody
    • RB binding protein 8 endonuclease antibody
    • RBBP-8 antibody
    • RBBP8 antibody
    • Retinoblastoma-binding protein 8 antibody
    • Retinoblastoma-interacting protein and myosin-like antibody
    • Rim antibody
    • SAE2 antibody
    • SCKL2 antibody
    • Sporulation in the absence of SPO11 protein 2 homolog antibody
    see all

Images

  • All lanes : Anti-CtIP (phospho S738) antibody [EP18893] (ab195291) at 1/1000 dilution

    Lane 1 : Untreated HEK-293 (human epithelial cell line from embryonic kidney) cultivated in RPMI 1640 media containing 10% FBS for 2 hours, whole cell lysate
    Lane 2 : HEK-293 treated with 60 J/m² UV light, then cultivated in RPMI 1640 media containing 10% FBS for 2 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Developed using the ECL technique

    Predicted band size : 102 kDa
    Observed band size : 102 kDa


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Dot blot analysis of CtIP (phospho S738) labeled with ab195291 at 1/1000 dilution.

    Lane 1: CtIP (phospho S738) peptide;

    Lane 2: CtIP non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

References

ab195291 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab195291.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up