Overview

  • Product nameAnti-Ctip1 antibody [14B5]
    See all Ctip1 primary antibodies
  • Description
    Mouse monoclonal [14B5] to Ctip1
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ICC, IP, IHC (PFA fixed), ChIP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human, Zebrafish
  • Immunogen

    A cocktail of BCL11A fusion proteins.

  • EpitopeEpitope is in core of CTIP1/Bcl11a (aa's 172-434).

Properties

Applications

Our Abpromise guarantee covers the use of ab19487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC (PFA fixed) Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 23576758
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionFunctions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1.
  • Tissue specificityExpressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.
  • Involvement in diseaseNote=Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification.
  • Sequence similaritiesContains 6 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Sumoylated by SUMO1.
  • Cellular localizationCytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810047E18Rik antibody
    • B cell CLL/lymphoma 11A (zinc finger protein) antibody
    • B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody
    • B-cell CLL/lymphoma 11A antibody
    • B-cell lymphoma/leukemia 11A antibody
    • BC11A_HUMAN antibody
    • BCL-11A antibody
    • BCL11A antibody
    • BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1 antibody
    • BCL11A L antibody
    • BCL11A S antibody
    • BCL11A XL antibody
    • BCL11a-M antibody
    • BCL11AL antibody
    • BCL11AS antibody
    • BCL11AXL antibody
    • C2H2 type zinc finger protein antibody
    • COUP TF interacting protein 1 antibody
    • COUP-TF-interacting protein 1 antibody
    • CTIP1 antibody
    • CTIP1, mouse, homolog of antibody
    • D930021L15Rik antibody
    • Ecotropic viral integration site 9 antibody
    • Ecotropic viral integration site 9 homolog antibody
    • Ecotropic viral integration site 9 protein antibody
    • Ecotropic viral integration site 9 protein homolog antibody
    • EVI-9 antibody
    • Evi9 antibody
    • Evi9, mouse, homolog of antibody
    • FLJ10173 antibody
    • FLJ34997 antibody
    • HBFQTL5 antibody
    • KIAA1809 antibody
    • mKIAA1809 antibody
    • OTTHUMP00000159788 antibody
    • OTTHUMP00000159789 antibody
    • OTTHUMP00000201250 antibody
    • OTTHUMP00000202084 antibody
    • Zinc finger protein 856 antibody
    • ZNF856 antibody
    see all

Anti-Ctip1 antibody [14B5] images

  • Overlay histogram showing Ramos cells stained with ab19487 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19487, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-Ctip1 antibody [14B5] (ab19487)

    Lane 1 : C6 Cell Line
    Lane 2 : MEF Cell Line
    Lane 3 : HDFn Cell LIne
    Lane 4 : Jurkat Cell Line
    Lane 5 : HEK293T Cell Line

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 91 kDa

    The CTIP1 protein band is indicated at 92 kDa. This antibody also identified a 55 kDa protein band in Western blotting, principally in mouse fibroblasts (MEF), but this is unrelated to.

     

    Blocking was performed in 5% milk (in PBS). The primary antibody was diluted in 1% milk (in PBS) and incubated overnight at 4ºC.

  • Anti-Ctip1 antibody [14B5] (ab19487) at 1 µg/ml + Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 91 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,42 kDa,62 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
    The 120 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Ctip1.

References for Anti-Ctip1 antibody [14B5] (ab19487)

This product has been referenced in:
  • Xu J  et al. Corepressor-dependent silencing of fetal hemoglobin expression by BCL11A. Proc Natl Acad Sci U S A 110:6518-23 (2013). ChIP ; Human . Read more (PubMed: 23576758) »
  • Jiang BY  et al. BCL11A overexpression predicts survival and relapse in non-small cell lung cancer and is modulated by microRNA-30a and gene amplification. Mol Cancer 12:61 (2013). WB . Read more (PubMed: 23758992) »

See all 11 Publications for this product

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Thank you for your reply. I can confirm that the immunogens for this antibody were GST-CTIP1 1-171, GST-CTIP1 172-434 and GST-CTIP1 420-776. The three immunogens were used in approximately equal amount to immunize the mice. ab19487 was ...

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Thank you for your inquiry. All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. I can confirm his ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - nuclear (haematopoietic stem progenitor cell line)
Specification haematopoietic stem progenitor cell line
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.4% and 1% formaldehyde
Detection step Real-time PCR
Positive control Gata1 promoter and b-globin HS3
Username

Abcam user community

Verified customer

Submitted Oct 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA fixed)
Sample Mouse Tissue sections (Brain (P7 coronal section))
Specification Brain (P7 coronal section)
Username

Abcam user community

Verified customer

Submitted Feb 25 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"