Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 1-5µg/mg of lysate.
    WB: 1/1000 - 1/10000. Detects bands of approximately 80, 85 and 120 kDa, (predicted molecular weight of longest isoform: 91 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFunctions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1.
    • Tissue specificityExpressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.
    • Involvement in diseaseNote=Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification.
    • Sequence similaritiesContains 6 C2H2-type zinc fingers.
    • Post-translational
      modifications
      Sumoylated by SUMO1.
    • Cellular localizationCytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2810047E18Rik antibody
      • B cell CLL/lymphoma 11A (zinc finger protein) antibody
      • B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody
      • B-cell CLL/lymphoma 11A antibody
      • B-cell lymphoma/leukemia 11A antibody
      • BC11A_HUMAN antibody
      • BCL-11A antibody
      • BCL11A antibody
      • BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1 antibody
      • BCL11A L antibody
      • BCL11A S antibody
      • BCL11A XL antibody
      • BCL11a-M antibody
      • BCL11AL antibody
      • BCL11AS antibody
      • BCL11AXL antibody
      • C2H2 type zinc finger protein antibody
      • COUP TF interacting protein 1 antibody
      • COUP-TF-interacting protein 1 antibody
      • CTIP1 antibody
      • CTIP1, mouse, homolog of antibody
      • D930021L15Rik antibody
      • Ecotropic viral integration site 9 antibody
      • Ecotropic viral integration site 9 homolog antibody
      • Ecotropic viral integration site 9 protein antibody
      • Ecotropic viral integration site 9 protein homolog antibody
      • EVI-9 antibody
      • Evi9 antibody
      • Evi9, mouse, homolog of antibody
      • FLJ10173 antibody
      • FLJ34997 antibody
      • HBFQTL5 antibody
      • KIAA1809 antibody
      • mKIAA1809 antibody
      • OTTHUMP00000159788 antibody
      • OTTHUMP00000159789 antibody
      • OTTHUMP00000201250 antibody
      • OTTHUMP00000202084 antibody
      • Zinc finger protein 856 antibody
      • ZNF856 antibody
      see all

    Anti-Ctip1 antibody images

    • Lane 1 : Anti-Ctip1 antibody (ab72247) at 1 µg/ml
      Lane 2 : Anti-Ctip1 antibody (ab72247) at 0.1 µg/ml

      Lane 1 : Nuclear extract from Raji cells
      Lane 2 : Nuclear extract from Raji cells

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 91 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 80 kDa,85 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of Ctip1.
      Lane 1: ab72247 at 1µg/0.5mg lysate for IP.
      ab72247 at 0.5µg/ml for WB.
      Nuclear lysate from Raji cells at 0.5mg for IP.
      Detection:Chemiluminescence with an exposure time of 5 minutes.

    References for Anti-Ctip1 antibody (ab72247)

    ab72247 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"