Overview

  • Product nameAnti-Ctip2 antibody
    See all Ctip2 primary antibodies
  • Description
    Rabbit polyclonal to Ctip2
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residues 500 and 550 of human Ctip2 (Swiss-Prot Q9C0K0).

  • Positive control
    • Nuclear extract from Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab70452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000 - 1/15000. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa).
IP Use at 1-5 µg/mg of lysate.

Target

  • FunctionTumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
  • Tissue specificityHighly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • Sequence similaritiesContains 6 C2H2-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATL1 alpha antibody
    • ATL1 antibody
    • ATL1 beta antibody
    • ATL1 gamma antibody
    • ATL1-delta antibody
    • B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein antibody
    • B cell lymphoma/leukemia 11B antibody
    • B-cell CLL/lymphoma 11B (zinc finger protein) antibody
    • B-cell CLL/lymphoma 11B antibody
    • B-cell lymphoma/leukemia 11B antibody
    • BC11B_HUMAN antibody
    • BCL-11B antibody
    • Bcl11b antibody
    • BCL11B/TRDC fusion antibody
    • COUP TF interacting protein 2 antibody
    • COUP-TF interacting protein 2 antibody
    • COUP-TF-interacting protein 2 antibody
    • Ctip 2 antibody
    • CTIP-2 antibody
    • CTIP2 antibody
    • hRIT1 alpha antibody
    • hRit1 antibody
    • Radiation induced tumor suppressor gene 1 antibody
    • Radiation induced tumor suppressor gene 1 protein antibody
    • Radiation-induced tumor suppressor gene 1 protein antibody
    • Rit 1 antibody
    • Rit1 antibody
    • zinc finger protein hRit1 alpha antibody
    • ZNF856B antibody
    see all

Anti-Ctip2 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling Ctip2 with ab70452 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Ctip2 antibody (ab70452) at 0.1 µg/ml

    Lane 1 : Jurkat cell nuclear extract at 5 µg
    Lane 2 : Jurkat cell nuclear extract at 50 µg


    Predicted band size : 99 kDa
    Observed band size : 120-130 kDa (why is the actual band size different from the predicted?)
  • All lanes: 1mg of nuclear extract from Jurkat cell treated with ab70452 at 0.5µg/ml.
    Lane 1: ab70452 used at 1 µg/mg lysate for IP.
    Lane 2: ab70452 used at 10 µg/mg lysate for IP.
    Lane 3: an alternate Ctip2 antibody (ab70453) used at 1 µg/mg lysate for IP.
    Lane 4: an alternate Ctip2 antibody (ab70453) used at 10 µg/mg lysate for IP.
    Normal rabbit IgG was used as a control for immunoprecipitation reactions. Detection:
    Chemiluminescence with exposure time of 30 seconds.

References for Anti-Ctip2 antibody (ab70452)

ab70452 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"