The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/5000 - 1/15000. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa).
Use at 1-5 µg/mg of lysate.
FunctionTumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
Tissue specificityHighly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
All lanes: 1mg of nuclear extract from Jurkat cell treated with ab70452 at 0.5µg/ml.
Lane 1: ab70452 used at 1 µg/mg lysate for IP.
Lane 2: ab70452 used at 10 µg/mg lysate for IP.
Lane 3: an alternate Ctip2 antibody (ab70453) used at 1 µg/mg lysate for IP.
Lane 4: an alternate Ctip2 antibody (ab70453) used at 10 µg/mg lysate for IP.
Normal rabbit IgG was used as a control for immunoprecipitation reactions.
Chemiluminescence with exposure time of 30 seconds.
References for Anti-Ctip2 antibody (ab70452)
has not yet been referenced specifically in any publications.
Publishing research using ab70452? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"