The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/15000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 100 kDa).
Use at 1-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionTumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
Tissue specificityHighly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Ctip2 with ab70453 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Ctip2 antibody (ab70453)
All lanes: 1mg nuclear extract from Jurkat cells analysed on a blot using ab70453 at 0.5µg/ml.
Lane 1: an alternate Ctip2 antibody used at 1 µg/mg lysate for IP.
Lane 2: The same alternate antibody used at 10 µg/mg lysate for IP.
Lane 3: ab70453 used at 1 µg/mg lysate for IP.
Lane 4: ab70453 at 10 µg/mg lysate for IP.
Normal rabbit IgG was used as a control for immunoprecipitation reactions.
Western blot - Ctip2 antibody (ab70453)
All lanes : Anti-Ctip2 antibody (ab70453) at 0.1 µg/ml
Lane 1 : Jurkat cell nuclear extract at 5 µg Lane 2 : Jurkat cell nuclear extract at 50 µg