Overview

  • Product name
    Anti-Ctk1 antibody
  • Description
    Rabbit polyclonal to Ctk1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Saccharomyces cerevisiae Ctk1.

    (Peptide available as ab32250.)

  • Positive control
    • This antibody gave a positive signal in S.cerevisiae (Y190) Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab47812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 60 kDa).

Target

  • Relevance
    Ctk1 is the catalytic subunit of CTDK-1 that hyperphosphorylates the C-terminal repeat domain (CTD) of RNA polymerase II largest subunit. CTDK-I is localized to the nucleolus. Ctk1 also co-precipitates with RNA polymerase I co-indicating a direct interaction between RNA polymerase I and Ctk1. Therefore, CTDK-I appears to play a role regulating distinct nuclear transcriptional machineries.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • CTD kinase 58 kDa subunit antibody
      • CTD kinase subunit 1 antibody
      • CTD kinase subunit alpha antibody
      • CTDK I alpha subunit antibody

    Images

    • Anti-Ctk1 antibody (ab47812) at 1 µg/ml + S.cerevisiae (Y190) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 60 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 110 kDa,120 kDa,25 kDa,65 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 8 minutes

    References

    ab47812 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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