Overview

  • Product nameCTLA4 (CD80) Mouse ELISA Kit
    See all CD80 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Other biological fluids, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    62.5 pg/ml - 4000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s mouse CTLA4 (CD80) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse CTLA4 in cell culture supernatants and serum.

    A CTLA4 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for CTLA4 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse CTLA4 amount of sample captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionInvolved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • Tissue specificityExpressed on activated B-cells, macrophages and dendritic cells.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Alternative names
    • Activation B7-1 antigen
    • B7
    • BB1
    • CD80
    • CD80_HUMAN
    • CTLA-4 counter-receptor B7.1
    • T-lymphocyte activation antigen CD80
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

CTLA4 (CD80) Mouse ELISA Kit images

  • Representative Standard Curve Using ab119578.

Protocols

References for CTLA4 (CD80) Mouse ELISA Kit (ab119578)

ab119578 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119578.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"