Overview

  • Product name
  • Description
    Rabbit polyclonal to CTNNBL1
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MDVGELLSYQ PNRGTKRPRD DEEEEQKMRR KQTGTRERGR YREEEMTVVE) of Human CTNNBL1 (NP_110517.2).

  • Positive control
    • HeLa, 293T and NIH-3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab95170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Induces apoptosis in CHO cells.
  • Tissue specificity
    Widely expressed with highest levels in skeletal muscle, placenta, heart, spleen, testis and thyroid.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5730471K09Rik antibody
    • Beta catenin like 1 antibody
    • Beta catenin like protein 1 antibody
    • Beta-catenin-like protein 1 antibody
    • C20orf33 antibody
    • Cadherin associated protein antibody
    • Catenin beta like 1 antibody
    • Chromosome 20 open reading frame 3 antibody
    • CTBL1_HUMAN antibody
    • CTNN protein antibody
    • CTNNBL 1 antibody
    • Ctnnbl1 antibody
    • dJ633O20.1 antibody
    • FLJ21108 antibody
    • NAP antibody
    • Nuclear associated protein antibody
    • nuclear protein NAP antibody
    • Nuclear-associated protein antibody
    • NYD SP19 antibody
    • OTTMUSP00000016933 antibody
    • P14 antibody
    • P14L antibody
    • PP8304 antibody
    • RP5 1118M15.1 antibody
    • Testis development protein NYD SP19 antibody
    • Testis development protein NYD-SP19 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling CTNNBL1 with ab95170 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CTNNBL1 antibody (ab95170) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 65 kDa


    Exposure time : 3 minutes
  • Detection of CTNNBL1 in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab95170 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with exposure time of 3 minutes.

References

ab95170 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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