Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling CTNNBL1 with ab95170 at 1/200 (1µg/ml). Detection: DAB.
Western blot - CTNNBL1 antibody (ab95170)
All lanes : Anti-CTNNBL1 antibody (ab95170) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 65 kDa
Exposure time : 3 minutes
Immunoprecipitation - CTNNBL1 antibody (ab95170)
Detection of CTNNBL1 in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab95170 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with exposure time of 3 minutes.
References for Anti-CTNNBL1 antibody (ab95170)
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