Overview

  • Product nameAnti-CTR9 antibody
    See all CTR9 primary antibodies
  • Description
    Rabbit polyclonal to CTR9
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to amino acids 1123-1173 of Human Paf1/RNA polymerase II complex component homolog CTR9 using the numbering given in entry NP_055448.1

  • Positive control
    • Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Lung Adenocarcinoma, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Stomach Adenocarcinoma, Testicular Seminoma. Mouse controls: Colon Carcinoma CT26, Teratoma

Properties

Applications

Our Abpromise guarantee covers the use of ab84487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Antigen retrieval is recommended: retrieval with citrate buffer will enhance staining. Likely to work with frozen sections
WB Use at an assay dependent concentration. Predicted molecular weight: 133.5 kDa.
ICC/IF 1/250.

Target

  • Tissue specificityWidely expressed.
  • Sequence similaritiesContains 16 TPR repeats.
  • Cellular localizationNucleus speckle. Found in speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ctr 9 antibody
    • CTR9 antibody
    • Ctr9 Paf1/RNA polymerase II complex component, homolog antibody
    • CTR9, Paf1/RNA polymerase II complex componen antibody
    • CTR9, S. cerevisiae, homolog of antibody
    • CTR9_HUMAN antibody
    • KIAA0155 antibody
    • p150 antibody
    • p150(TSP) antibody
    • p150TSP. antibody
    • Paf1/RNA polymerase II complex component homolog Ctr9 antibody
    • RNA polymerase associated protein CTR9 homolog antibody
    • RNA polymerase-associated protein CTR9 homolog antibody
    • SH2 binding phosphoprotein antibody
    • SH2 domain binding protein 1 (tetratricopeptide repeat containing) antibody
    • SH2 domain binding protein 1 antibody
    • SH2 domain-binding protein 1 antibody
    • SH2-binding phosphoprotein of 150 kDa antibody
    • SH2BP1 antibody
    • Tetratricopeptide repeat-containing antibody
    • Tetratricopeptide-containing SH2-binding phosphoprotein 150-KD antibody
    • TPR containing, SH2-binding phosphoprotein of 150 kDa antibody
    • TSBP antibody
    see all

Anti-CTR9 antibody images

  • ab84487 at 1/250 dilution staining formalin-fixed, paraffin-embedded section of human breast carcinoma by immunohistochemistry.
    Detection: DAB staining.
  • ab 84487 at 1/250 dilution staining formalin-fixed, paraffin-embedded section of human breast carcinoma by immunohistochemistry.
    Detection: DAB staining
  • ab 84487 at 1/250 dilution staining formalin-fixed, paraffin-embedded section of mouse teratoma by immunohistochemistry.
    Detection: DAB staining
  • ab84487 staining CTR9 in Mouse ES cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 0.2% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/250 PBS + 0.2% fish scale gelatin in PBS) for 20 minutes at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-CTR9 antibody (ab84487)

This product has been referenced in:
  • Zhang K  et al. CTR9/PAF1c regulates molecular lineage identity, histone H3K36 trimethylation and genomic imprinting during preimplantation development. Dev Biol 383:15-27 (2013). Read more (PubMed: 24036311) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse ES cells)
Loading amount 100000 cells
Specification mouse ES cells
Treatment 72 hours after transfection with Silencer® Select Pre-designed siRNA against Ctr9 (s75508)
Gel Running Conditions Non-reduced Denaturing (Invitrogen NuPage 4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse ES cells)
Specification mouse ES cells
Fixative Methanol
Permeabilization No
Blocking step Fish scale gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"