Anti-CTRB1 antibody (ab86177)

Overview

  • Product name
  • Description
    Rabbit polyclonal to CTRB1
  • Specificity
    ab86177 recognizes both forms of this proteolytic enzyme.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide from within a region of internal sequence amino acids 107-156 (KNPKFSILTV NNDITLLKLA TPARFSQTVS AVCLPSADDD FPAGTLCATT) of Human CTRB1 (NP_001897)

  • Positive control
    • Human fetal muscle lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    CTRB1 (Chymotrypsinogen B1) is the inactive precursor of Alpha chymotrypsin, one of a family of serine proteases secreted into the gastrointestinal tract. The zymogen is activated by proteolytic cleavage by trypsin.
  • Cellular localization
    Secreted, extracellular space.
  • Database links
  • Alternative names
    • Chymotrypsinogen B antibody
    • Chymotrypsinogen B1 antibody
    • ChymotrypsinogenB antibody
    • ChymotrypsinogenB1 antibody
    • CTRB 1 antibody
    • CTRB antibody
    • FLJ42412 antibody
    • MGC88037 antibody
    • OTTHUMP00000214638 antibody
    see all

Anti-CTRB1 antibody images

  • Anti-CTRB1 antibody (ab86177) at 1 µg/ml (5% skim milk/ PBS buffer) + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa

References for Anti-CTRB1 antibody (ab86177)

ab86177 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Serum (serum (with pancreatic tumor))
Loading amount
30 µg
Specification
serum (with pancreatic tumor)
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 24 2012

Thank you for your inquiry.

Unfortunately, I am unable to issue discount code for the following antibodies:

ab60720, ab86704 and ab65109.

The immunogens for the MUC antibodies do not show a high enough homology (70% and be...

Read More

Thank you for your enquiry and your interest. Although, ab86177 has not been tested on either chymotrypsin or chymotypsinogen - only in samples which contain the precursor and the cleaved forms - based on the peptide immunogen used to raise this antibo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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