Overview

  • Product nameAnti-CTRP1 antibody
    See all CTRP1 primary antibodies
  • Description
    Rabbit polyclonal to CTRP1
  • Specificityab25973 recognises CTRP1 (G protein coupled receptor interacting protein 1, GIP).
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, consisting of 15 N terminal amino acids of Human CTRP1.

  • Positive control
    • Human kidney cell lysate

Associated products

Applications

Our Abpromise guarantee covers the use of ab25973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
IHC-P
ICC/IF
WB
  • Application notesIHC-P: Use at a concentration of 10 µg/ml.
    IHC-Fr: Use at an assay dependent concentration (See Abreview).
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. Can be blocked with CTRP1 peptide (ab39751).
    Note: CTRP1 is highly modified post-translationally and can migrate in SDS-PAGE at positions other than their predicted size.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-CTRP1 antibody images

    • Lane 1 : Anti-CTRP1 antibody (ab25973) at 1 µg/ml
      Lane 2 : Anti-CTRP1 antibody (ab25973) at 2 µg/ml

      Lane 1 : Human kidney cell lysate
      Lane 2 : Human kidney cell lysate


      Predicted band size : 32 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    • ab25973 at 10µg/ml staining CTRP1 in human kidney tissue by IHC 

    • ab27973 staining CTRP1 in rat vibrissa tissue sections by Immunohistochemistry (frozen sections). Tissue was un-fixed but permeabilized with 0.1% Triton X in PBS. Samples were then blocked with 10% serum for 30 minutes at 25°C followed by incubation with the primary antibody, at a 1/100 dilution, for 15 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG (H+L) was used as secondary antibody at a 1/500 dilution.

      See Abreview

    • Immunofluorescence of CTRP1 in Human Kidney cells using ab25973 at 20 ug/ml.

    References for Anti-CTRP1 antibody (ab25973)

    ab25973 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Vibrissa)
    Specification Vibrissa
    Fixative No fixative
    Permeabilization Yes - 0.1% Triton X in PBS
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Ms. Nicola Broughton

    Verified customer

    Submitted Mar 29 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"