The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). This protein is often highly modified post-translationally and migrates in SDS-PAGE at positions other than its predicted size.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.