Overview

  • Product nameAnti-CTRP4 antibody
    See all CTRP4 primary antibodies
  • Description
    Rabbit polyclonal to CTRP4
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid peptide from near the carboxy terminus of human CTRP4.

  • Positive control
    • Rat brain cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab36871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).Can be blocked with Human CTRP4 peptide (ab39741).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • RelevanceAdipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. Multiple isoforms of mouse CTRP4 have been reported.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • C1q and tumor necrosis factor related protein 4 antibody
    • C1QTNF4 antibody
    • Complement C1q tumor necrosis factor related protein antibody
    • ZACRP4 antibody

Anti-CTRP4 antibody images

  • Lane 1 : Anti-CTRP4 antibody (ab36871) at 1 µg/ml
    Lane 2 : Anti-CTRP4 antibody (ab36871) at 2 µg/ml
    Lane 3 : Anti-CTRP4 antibody (ab36871) at 4 µg/ml

    Lane 1 : Rat brain cell lysate
    Lane 2 : Rat brain cell lysate
    Lane 3 : Rat brain cell lysate

    Lysates/proteins at 15 µg/ml per lane.

    Secondary
    anti-rabbit IgG HRP at 1/10000 dilution

    Predicted band size : 37 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Lanes read from left to right 1, 2 and 3. These proteins are often highly modified post-translationally and may migrate in SDS-PAGE at positions other than their predicted size.
  • ab36891 at 10µg/ml staining CTRP4 in rat brain by IHC

  • Immunofluorescence of CTRP4 in Rat Brain cells using ab36871 at 20 ug/ml.

References for Anti-CTRP4 antibody (ab36871)

This product has been referenced in:
  • Byerly MS  et al. C1q/TNF-related protein 4 (CTRP4) is a unique secreted protein with two tandem C1q domains that functions in the hypothalamus to modulate food intake and body weight. J Biol Chem 289:4055-69 (2014). WB ; Mouse . Read more (PubMed: 24366864) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab36871.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"