The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 25 kDa).Can be blocked with Human CTRP5 peptide (ab39744).
Use a concentration of 10 µg/ml.
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. CTRP5 has been suggested to be involved in age-related macular degeneration.
C1q and tumor necrosis factor related protein 5 antibody
Complement C1q tumor necrosis factor related protein 5 precursor antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP5 antibody (ab36893)This image is courtesy of an Abreview submitted by Steffen Rickelt
ab36893 staining CTRP5 in human pancreas carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in 10mM sodium citrate + 0.05% Tween. Samples were incubated with primary antibody (1/750 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Lane 1 : Anti-CTRP5 antibody (ab36893) at 1 µg/ml Lane 2 : Anti-CTRP5 antibody (ab36893) at 2 µg/ml Lane 3 : Anti-CTRP5 antibody (ab36893) at 4 µg/ml
Lane 1 : Human brain cell lysate Lane 2 : Human brain cell lysate Lane 3 : Human brain cell lysate
Lysates/proteins at 15 µg/ml per lane.
Secondary Anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size : 25 kDa Observed band size : 39 kDa (why is the actual band size different from the predicted?) Lanes read from left to right, 1-3. These proteins are often highly modified post-translationally and may migrate in SDS-PAGE at positions other than their predicted size.