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antigen, corresponding to amino acids 56-82 (
PYSRMPASSWTDHKEALERGQFDYALV) of Human CTXN1.
Our Abpromise guarantee covers the use of ab121510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunocytochemistry/Immunofluorescence analysis of A-431 cells labelling CTXN1 with ab121510 shows positivity in plasma membrane, cell junctions & nucleus but is excluded from the nucleoli.
ab121510 has not yet been referenced specifically in any publications.