Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab65773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 460 kDa (predicted molecular weight: 399 kDa).

Target

  • FunctionCotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.
  • Tissue specificityExpressed in kidney proximal tubule cells, placenta, visceral yolk-sac cells and in absorptive intestinal cells. Expressed in the epithelium of intestine and kidney.
  • Involvement in diseaseRecessive hereditary megaloblastic anemia 1
  • Sequence similaritiesContains 27 CUB domains.
    Contains 7 EGF-like domains.
  • DomainThe CUB domains 5 to 8 mediate binding to GIF and ALB. CUB domains 1 and 2 mediate interaction with LRP2.
  • Post-translational
    modifications
    The precursor is cleaved by a trans-Golgi proteinase furin. The result is a propeptide cleaved off.
    N-glycosylated.
  • Cellular localizationEndosome membrane. Lysosome membrane. Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 460 kDa receptor antibody
    • cubilin (intrinsic factor-cobalamin receptor) antibody
    • Cubilin antibody
    • Cubilin precursor antibody
    • Cubn antibody
    • CUBN_HUMAN antibody
    • IFCR antibody
    • Intestinal intrinsic factor receptor antibody
    • intrinsic factor B12-receptor antibody
    • Intrinsic factor-cobalamin receptor antibody
    • Intrinsic factor-vitamin B12 receptor antibody
    • megaloblastic anemia 1 antibody
    • MGA1 antibody
    see all

Anti-Cubilin antibody images

  • Anti-Cubilin antibody (ab65773) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 399 kDa
    Observed band size : 460 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

References for Anti-Cubilin antibody (ab65773)

ab65773 has not yet been referenced specifically in any publications.

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