Anti-CUEDC2 antibody (ab109649)
Key features and details
- Rabbit polyclonal to CUEDC2
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CUEDC2 antibody
See all CUEDC2 primary antibodies -
Description
Rabbit polyclonal to CUEDC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
19 amino acid peptide from near the carboxy terminus of Human CUEDC2 (Q9H467)
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Positive control
- HeLa whole cell lysate (ab150035) Human spleen or tonsil tissue HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 32 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 32 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use at an assay dependent concentration. |
Target
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Function
Controls PGR and ESR1 protein levels through their targeting for ubiquitination and subsequent proteasomal degradation. -
Sequence similarities
Belongs to the CUEDC2 family.
Contains 1 CUE domain. -
Domain
The CUE domain mediates interaction with PGR and ESR1. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 79004 Human
- Entrez Gene: 67116 Mouse
- Entrez Gene: 294009 Rat
- Omim: 614142 Human
- SwissProt: Q9H467 Human
- SwissProt: Q9CXX9 Mouse
- SwissProt: A1L131 Rat
- Unigene: 500874 Human
see all -
Alternative names
- bA18I14.5 antibody
- C10orf66 antibody
- Chromosome 10 open reading frame 66 antibody
see all
Images
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Lane 1 : Anti-CUEDC2 antibody (ab109649) at 1 µg/ml
Lane 2 : Anti-CUEDC2 antibody (ab109649) at 2 µg/ml
All lanes : HeLa cell lysate
Predicted band size: 32 kDa -
ab109649, at 5µg/ml, staining Human CUEDC2 in spleen, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
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ab109649, at 5µg/ml, staining Human CUEDC2 in tonsil, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
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Immunocytochemistry image of HeLa cells, using ab109649 at 5 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab109649 has not yet been referenced specifically in any publications.