Overview

  • Product nameAnti-CUG-BP1 antibody
    See all CUG-BP1 primary antibodies
  • Description
    Rabbit polyclonal to CUG-BP1
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human CUG-BP1

  • Positive control
    • extracts from HeLa cells and HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab69583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA 1/40000.

Target

  • FunctionRNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the CELF/BRUNOL family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Phosphorylated. Its phosphorylation status increases in senescent cells.
  • Cellular localizationNucleus. Cytoplasm. RNA-binding activity is detected in both nuclear and cytoplasmic compartments.
  • Information by UniProt
  • Database links
  • Alternative names
    • 50 kDa Nuclear polyadenylated RNA binding protein antibody
    • 50 kDa nuclear polyadenylated RNA-binding protein antibody
    • Bruno like 2 antibody
    • bruno like protein 2 antibody
    • Bruno-like protein 2 antibody
    • BRUNOL 2 antibody
    • BRUNOL2 antibody
    • CELF 1 antibody
    • CELF-1 antibody
    • celf1 antibody
    • CELF1 CUGBP, Elav like family member 1 antibody
    • CELF1_HUMAN antibody
    • CUG BP and ETR 3 like factor 1 antibody
    • CUG BP antibody
    • CUG BP1 antibody
    • CUG RNA binding protein antibody
    • CUG triplet repeat RNA binding protein 1 antibody
    • CUG triplet repeat RNA-binding protein 1 antibody
    • CUG-BP antibody
    • CUG-BP- and ETR-3-like factor 1 antibody
    • CUG-BP1 antibody
    • CUGBP 1 antibody
    • CUGBP and ETR3 like factor 1 antibody
    • CUGBP antibody
    • CUGBP Elav like family member 1 antibody
    • CUGBP Elav-like family member 1 antibody
    • CUGBP1 antibody
    • Cytidine uridine guanosine binding protein 1 antibody
    • Deadenylation factor CUG BP antibody
    • Deadenylation factor CUG-BP antibody
    • Deadenylation factor CUGBP antibody
    • EDEN BP antibody
    • EDEN BP homolog antibody
    • EDEN-BP antibody
    • EDEN-BP homolog antibody
    • embryo deadenylation element binding protein antibody
    • embryo deadenylation element binding protein homolog antibody
    • Embryo deadenylation element-binding protein homolog antibody
    • hNab 50 antibody
    • hNab50 antibody
    • NAB 50 antibody
    • NAB50 antibody
    • NAPOR antibody
    • Nuclear polyadenylated RNA binding protein 50 kD antibody
    • Nuclear polyadenylated RNA binding protein antibody
    • RNA binding protein BRUNOL 2 antibody
    • RNA binding protein BRUNOL2 antibody
    • RNA-binding protein BRUNOL-2 antibody
    see all

Anti-CUG-BP1 antibody images

  • All lanes : Anti-CUG-BP1 antibody (ab69583) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from HepG2 cells
    Lane 3 : extracts from HeLa cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ICC/IF image of ab69583 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69583, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CUG-BP1 antibody (ab69583)

ab69583 has not yet been referenced specifically in any publications.

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