Anti-Cullin 1 antibody [EPR3102Y] (ab75812)


  • Product name
    Anti-Cullin 1 antibody [EPR3102Y]
    See all Cullin 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3102Y] to Cullin 1
  • Host species
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • 293T, A549 and T47D cell lysates; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


Associated products


Our Abpromise guarantee covers the use of ab75812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 90 kDa.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
    • Tissue specificity
      Expressed in lung fibroblasts.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the cullin family.
    • Post-translational
      Neddylated; which enhances the ubiquitination activity of SCF. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.
    • Information by UniProt
    • Database links
    • Alternative names
      • CUL 1 antibody
      • CUL-1 antibody
      • CUL1 antibody
      • CUL1_HUMAN antibody
      • Cullin-1 antibody
      • Cullin1 antibody
      • MGC149834 antibody
      • MGC149835 antibody
      see all


    • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Cullin 1 with ab75812 at 1/400 dilution. Cells were fixed in paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab75812 at 1/400 was carried in PBS buffer for 1 hour at 22°C. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody was used at 1/200 dilution. DAPI was used to counterstain.

      See Abreview

    • All lanes : Anti-Cullin 1 antibody [EPR3102Y] (ab75812) at 1/2000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : T47D cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 90 kDa
      Observed band size: 90 kDa

    • ab75812 at 1/100 dilution staining Cullin 1 in HeLa cells by Immunofluorescence.


    This product has been referenced in:
    • Bruckert WM  et al. Rapid nutritional remodeling of the host cell upon attachment of Legionella pneumophila. Infect Immun 82:72-82 (2014). Read more (PubMed: 24126522) »
    • Kainulainen M  et al. Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH. J Virol 88:3464-73 (2014). WB . Read more (PubMed: 24403578) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (HeLa)
    Yes - 0.5% Triton X-100 in PBS

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jan 16 2017


    Sign up