Overview

  • Product nameAnti-Cullin 2 antibody
    See all Cullin 2 primary antibodies
  • Description
    Mouse polyclonal to Cullin 2
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Orangutan
  • Immunogen

    Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 161-260 of Human Cullin 2. Target sequence used ot make the antibody: RMLLREIKND RGGEDPNQKV IHGVINSFVH VEQYKKKFPL KFYQEIFESP FLTETGEYYK QEASNLLQES NCSQYMEKVL GRLKDEEIRC RKYLHPSSYT.

Properties

Applications

Our Abpromise guarantee covers the use of ab43562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notesIP: Use at an assay dependent dilution (PMID 19622774).
    WB: 1/1000. Predicted molecular weight: 87 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCore component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the cullin family.
    • Post-translational
      modifications
      CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cul2 antibody
      • CUL 2 antibody
      • CUL-2 antibody
      • CUL2 antibody
      • CUL2_HUMAN antibody
      • cullin 2 antibody
      • cullin homolog 2 antibody
      • Cullin-2 antibody
      • MGC131970 antibody
      see all

    Anti-Cullin 2 antibody images

    • All lanes : Anti-Cullin 2 antibody (ab43562) at 1/1000 dilution

      Lane 1 : Total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
      Lane 2 : Total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein).

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM, HRP conjugated, at 1/5000 dilution

      Predicted band size : 87 kDa

    References for Anti-Cullin 2 antibody (ab43562)

    This product has been referenced in:
    • Ambrosini G  et al. Differentiation-related gene-1 decreases Bim stability by proteasome-mediated degradation. Cancer Res 69:6115-21 (2009). IP ; Human . Read more (PubMed: 19622774) »

    See 1 Publication for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"