The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notesIs unsuitable for WB.
FunctionCore component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the cullin family.
Post-translational modificationsCBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labelling Cullin 2 with ab90652 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Cullin 2 antibody (ab90652)
1mg whole cell lysate from HeLa cells was immunoprecipitated using 1µg ab90652. 20% of the immunoprecipitate was loaded in each lane, and probed with ab90652 at 1µg/ml (lane 1) or with a control IgG (lane 2). Detection: chemoluminescence, with an exposure time of 3 seconds.
References for Anti-Cullin 2 antibody (ab90652)
has not yet been referenced specifically in any publications.
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