FunctionCore component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the cullin family.
Post-translational modificationsCBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
1mg whole cell lysate from HeLa cells was immunoprecipitated using 1µg ab90653. 20% of the immunoprecipitate was loaded in each lane, and probed with ab90653 at 1µg/ml (lane 1) or with a control IgG (lane 2). Detection: chemoluminescence, with an exposure time of 3 seconds.
Western blot - Cullin 2 antibody (ab90653)
All lanes : Anti-Cullin 2 antibody (ab90653) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 87 kDa
References for Anti-Cullin 2 antibody (ab90653)
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