Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
Protein modification; protein ubiquitination.
Belongs to the cullin family.
CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
Flow cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Cullin 2 (red) with ab166917 at a 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Western blot analysis of an immunoprecipitation pellet from Raji cell lysate labeling Cullin 2 with ab166917 which was used at 1/10 dilution for immunoprecipitation. Secondary antibody used was an HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Western blot - Anti-Cullin 2 antibody [EPR3104(2)] (ab166917)
All lanes : Anti-Cullin 2 antibody [EPR3104(2)] (ab166917) at 1/2000 dilution
Lane 1 : Mouse brain lysate Lane 2 : Mouse heart lysate Lane 3 : Mouse kidney lysate Lane 4 : Mouse spleen lysate Lane 5 : Rat brain lysate Lane 6 : Rat heart lysate Lane 7 : Rat kidney lysate Lane 8 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution