Overview

  • Product name
  • Description
    Rabbit polyclonal to Cullin 3
  • Tested applications
    Suitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    MSNLSKGTGSRK

    conjugated to KLH, corresponding to amino acids 1-12 of Human Cullin 3

  • Positive control
    • Human breast tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab118960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 89 kDa.
IP Use at an assay dependent concentration.
ELISA 1/2000 - 1/10000.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition.
  • Tissue specificity
    Widely expressed.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the cullin family.
  • Post-translational
    modifications
    Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
  • Cellular localization
    Nucleus. Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CUL 3 antibody
    • Cul-3 antibody
    • cul3 antibody
    • CUL3_HUMAN antibody
    • Cullin-3 antibody
    • Cullin3 antibody
    • KIAA0617 antibody
    • PHA2E antibody
    see all

Images

  • ab118960 at 1/500 staining Cullin 3 in Human breast tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen

References

ab118960 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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