• Product nameAnti-Cullin 3 antibody
    See all Cullin 3 primary antibodies
  • Description
    Rabbit polyclonal to Cullin 3
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to amino acids 718-768 of Human Cullin 3 (NP_003581.1)

  • Positive control
    • HeLa whole cell lysate NIH3T3 whole cell lysate 293T whole cell lysate



Our Abpromise guarantee covers the use of ab72187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionCore component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition.
    • Tissue specificityWidely expressed.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the cullin family.
    • Post-translational
      Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
    • Cellular localizationNucleus. Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CUL 3 antibody
      • Cul-3 antibody
      • cul3 antibody
      • CUL3_HUMAN antibody
      • Cullin-3 antibody
      • Cullin3 antibody
      • KIAA0617 antibody
      • PHA2E antibody
      see all

    Anti-Cullin 3 antibody images

    • All lanes : Anti-Cullin 3 antibody (ab72187) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      developed using the ECL technique

      Predicted band size : 89 kDa
      Observed band size : 89 kDa

      Exposure time : 3 minutes

    References for Anti-Cullin 3 antibody (ab72187)

    This product has been referenced in:
    • Gastaldello S  et al. Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells. PLoS Pathog 9:e1003664 (2013). Human . Read more (PubMed: 24130483) »

    See 1 Publication for this product

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