Overview

  • Product nameAnti-Cullin 3 antibody [EPR3195]
    See all Cullin 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR3195] to Cullin 3
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Cullin 3.

  • Positive control
    • HeLa, SH-SY5Y, PC12 and NIH 3T3 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab108407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 89 kDa.
IP 1/50.
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionCore component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition.
    • Tissue specificityWidely expressed.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the cullin family.
    • Post-translational
      modifications
      Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
    • Cellular localizationNucleus. Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CUL 3 antibody
      • Cul-3 antibody
      • cul3 antibody
      • CUL3_HUMAN antibody
      • Cullin-3 antibody
      • Cullin3 antibody
      • KIAA0617 antibody
      • PHA2E antibody
      see all

    Anti-Cullin 3 antibody [EPR3195] images

    • All lanes : Anti-Cullin 3 antibody [EPR3195] (ab108407) at 1/5000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 89 kDa

    References for Anti-Cullin 3 antibody [EPR3195] (ab108407)

    This product has been referenced in:
    • Lee J  et al. Autophagy suppresses interleukin-1ß (IL-1ß) signaling by activation of p62 degradation via lysosomal and proteasomal pathways. J Biol Chem 287:4033-40 (2012). IP . Read more (PubMed: 22167182) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"