Recombinant
RabMAb

Anti-Cullin 3 antibody [EPR3196Y] (ab75851)

Overview

  • Product name
    Anti-Cullin 3 antibody [EPR3196Y]
    See all Cullin 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR3196Y] to Cullin 3
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C terminus of human Cullin 3.

  • Positive control
    • HeLa, SH-SY5Y, PC12 and 3T3 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab75851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000 - 1/100000. Predicted molecular weight: 89 kDa.
IP 1/40.
Flow Cyt 1/30 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition.
    • Tissue specificity
      Widely expressed.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the cullin family.
    • Post-translational
      modifications
      Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
    • Cellular localization
      Nucleus. Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CUL 3 antibody
      • Cul-3 antibody
      • cul3 antibody
      • CUL3_HUMAN antibody
      • Cullin-3 antibody
      • Cullin3 antibody
      • KIAA0617 antibody
      • PHA2E antibody
      see all

    Images

    • Overlay histogram showing HeLa cells stained with ab75851 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75851, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Cullin 3 antibody [EPR3196Y] (ab75851) at 1/20000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 89 kDa
      Observed band size : 82,90 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Jimenez-Blasco D  et al. Astrocyte NMDA receptors' activity sustains neuronal survival through a Cdk5-Nrf2 pathway. Cell Death Differ 22:1877-89 (2015). WB ; Rat . Read more (PubMed: 25909891) »
    • Zhang T  et al. G-protein-coupled receptors regulate autophagy by ZBTB16-mediated ubiquitination and proteasomal degradation of Atg14L. Elife 4:e06734 (2015). Human . Read more (PubMed: 25821988) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    No
    Specification
    HeLa
    Fixative
    Methanol
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 06 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (THP-1)
    Loading amount
    10 µg
    Specification
    THP-1
    Gel Running Conditions
    Reduced Denaturing (5-20% gradient)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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