• Product name
  • Description
    Rabbit polyclonal to Cullin 4a
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Chicken, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Sea urchin, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal residues 700 - 759) of Human Cullin 4a (NP_001008895.1)

  • Positive control
    • Human breast tumor; Mouse squamous cell carcinoma.



Our Abpromise guarantee covers the use of ab84382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure with citrate buffer will enhance staining.
    Note: expected to work on frozen secions.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the cullin family.
    • Post-translational
      Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity). Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2810470J21Rik antibody
      • AW495282 antibody
      • CUL 4A antibody
      • CUL-4A antibody
      • Cul4a antibody
      • Cul4a protein antibody
      • CUL4A_HUMAN antibody
      • Cullin-4A antibody
      • MGC36573 antibody
      • MGC64071 antibody
      see all


    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast tumor tissue using 1/500 ab84382 followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse squamous cell carcinoma tissue using 1/100 ab84382, followed by DAB staining.


    This product has been referenced in:
    • Ren S  et al. Oncogenic CUL4A determines the response to thalidomide treatment in prostate cancer. J Mol Med (Berl) 90:1121-32 (2012). Read more (PubMed: 22422151) »

    See 1 Publication for this product

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