Anti-Cullin 4A/B antibody [EPR3200] (ab76470)

Overview

  • Product nameAnti-Cullin 4A/B antibody [EPR3200]
  • Description
    Rabbit monoclonal [EPR3200] to Cullin 4A/B
  • SpecificityDue to the close homology between Cullin 4A and 4B this antibody is likely to react with both forms.
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in human Cullin 4B

  • Positive control
    • Fetal uterus, HeLa, A375 and fetal testis lysates and human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab76470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 88, 102 kDa (predicted molecular weight: 102 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.

Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC/IF or IP.
  • Target

    • RelevanceCore component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component.
    • Database links

    Anti-Cullin 4A/B antibody [EPR3200] images

    • All lanes : Anti-Cullin 4A/B antibody [EPR3200] (ab76470) at 1/5000 dilution

      Lane 1 : Fetal uterus lysate
      Lane 2 : Hela lysate
      Lane 3 : A375 lysate
      Lane 4 : Fetal testis lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 102 kDa
      Observed band size : 88,102 kDa (why is the actual band size different from the predicted?)
    • ab76470 at 1/100 dilution staining Cullin 4B in human brain tissue, using a HRP/AP polymerized secondary antibody.

    References for Anti-Cullin 4A/B antibody [EPR3200] (ab76470)

    ab76470 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry regading ab76470.
    I can confirm that the sequence we used to raise Anti-Cullin 4A/B antibody maps to a region that is conserved between CUL4A and B.
    In addition, we observe 2 bands (at 88 and at 102 kDa) in samples ...

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    Thank you for your inquiry.
    Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.
    We aim to provide as much information as possible to our customers, so I am sorry that...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"