Overview

  • Product name
  • Description
    Mouse polyclonal to Cullin 4B
  • Tested applications
    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Cullin 4B (NP_003579, 1 a.a. ~ 913 a.a) full length protein (human).

  • Positive control
    • WB: Transfected 293T cell lysate ICC/IF: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab67035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    Note: ab67035 requires Protein A affinity purification prior to ICC/IF application.
    WB: 1/500 - 1/1000. Detects a band of approximately 99 kDa (predicted molecular weight: 103 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8.
    • Pathway
      Protein modification; protein ubiquitination.
    • Involvement in disease
      Defects in CUL4B are the cause of mental retardation syndromic X-linked Cabezas type (MRXC) [MIM:300354]; also known as mental retardation syndromic X-linked type 15. A syndromic form of X-linked mental retardation characterized by severe intellectual deficit associated with short stature, craniofacial dysmorphism, small testes, muscle wasting in lower legs, kyphosis, joint hyperextensibility, pes cavus, small feet, and abnormalities of the toes. Additional neurologic manifestations include speech delay and impairment, tremor, seizures, gait ataxia, hyperactivity and decreased attention span.
    • Sequence similarities
      Belongs to the cullin family.
    • Post-translational
      modifications
      Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CUL 4B antibody
      • CUL-4B antibody
      • CUL4B antibody
      • CUL4B_HUMAN antibody
      • Cullin-4B antibody
      • DKFZp686F1470 antibody
      • KIAA0695 antibody
      • MRXHF2 antibody
      • MRXSC antibody
      • SFM2 antibody
      see all

    Images

    • All lanes : Anti-Cullin 4B antibody (ab67035) at 1/500 dilution

      Lane 1 : Transfected 293T cell lysate
      Lane 2 : non Transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 103 kDa
      Observed band size : 99 kDa (why is the actual band size different from the predicted?)
    • ab67035, at a concentration 10 µg/ml, staining Cullin 4B in HeLa cell by Immunofluorescence.
    • All lanes : Anti-Cullin 4B antibody (ab67035) at 1/500 dilution (Blocker 1:1 in PBS + 0.1% Tween for 14 hours at 4°C)

      Lane 1 : HeLa whole cell lysate
      Lane 2 : Cul4 siRNA in HeLa whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      An Alexa Fluor®680-conjugated Goat anti-mouse polyclonal at 1/10000 dilution

      Predicted band size : 103 kDa

      This image is courtesy of an Abreview submitted by Svetlana Khoronenkova

      See Abreview

    References

    This product has been referenced in:
    • Zou Y  et al. Characterization of nuclear localization signal in the N terminus of CUL4B and its essential role in cyclin E degradation and cell cycle progression. J Biol Chem 284:33320-32 (2009). WB, ICC/IF ; Human . Read more (PubMed: 19801544) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Sample
    Human Cell (Human primary fibroblasts)
    Specification
    Human primary fibroblasts
    Permeabilization
    Yes - 0.2% Triton-X100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 30 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Loading amount
    50 µg
    Specification
    Mouse embryonic fibroblasts
    Gel Running Conditions
    Reduced Denaturing (4-16% Tris-Gly gel)
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 26 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Total protein in input
    200 µg
    Specification
    HeLa cells
    Immuno-precipitation step
    Protein G
    Username

    Abcam user community

    Verified customer

    Submitted Jul 13 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    30 µg
    Specification
    HeLa cells
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Gly)
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Username

    Dr. Svetlana Khoronenkova

    Verified customer

    Submitted Aug 06 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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