Overview

  • Product name
  • Description
    Rabbit polyclonal to Cullin 5
  • Specificity
    This antibody reacts with human Cullin 5. It may cross react with other human cullins. It also co-immunoprecipitates proteins associated with Cullin 5.
  • Tested applications
    Suitable for: WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Zebrafish
  • Immunogen

    Synthetic peptide:

    SNLLKNK GSLQFEDK

    conjugated to KLH, corresponding to N terminal amino acids 4-18 of Human Cullin 5

  • Positive control
    • 293T and U2OS cell lysates. Most cell lines expressing Cul4A.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Purification notes
    This product is prepared by delipidation and defibrination followed by sterile filtration.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 91 kDa.
ELISA 1/2000 - 1/10000.
IP Use at an assay dependent dilution.

Target

  • Function
    Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the cullin family.
  • Post-translational
    modifications
    Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN).
  • Information by UniProt
  • Database links
  • Alternative names
    • CUL 5 antibody
    • CUL-5 antibody
    • CUL5 antibody
    • CUL5_HUMAN antibody
    • Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1) antibody
    • Cullin-5 antibody
    • Cullin5 antibody
    • VACM 1 antibody
    • VACM-1 antibody
    • VACM1 antibody
    • Vasopressin activated calcium mobilizing receptor 1 antibody
    • Vasopressin activated calcium mobilizing receptor antibody
    • Vasopressin-activated calcium-mobilizing receptor 1 antibody
    • Vasopressin-activated calcium-mobilizing receptor-1 antibody
    see all

References

ab34840 has not yet been referenced specifically in any publications.

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