Anti-Cullin 5 antibody - Carboxyterminal end (ab82292)

Overview

  • Product name
    Anti-Cullin 5 antibody - Carboxyterminal end
    See all Cullin 5 primary antibodies
  • Description
    Rabbit polyclonal to Cullin 5 - Carboxyterminal end
  • Tested applications
    Suitable for: WB, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide selected from the C terminal region of Human Cullin 5, conjugated to KLH

  • Positive control
    • Hela cell line lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 91 kDa.
Indirect ELISA 1/1000.

Target

  • Function
    Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the cullin family.
  • Post-translational
    modifications
    Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN).
  • Information by UniProt
  • Database links
  • Alternative names
    • CUL 5 antibody
    • CUL-5 antibody
    • CUL5 antibody
    • CUL5_HUMAN antibody
    • Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1) antibody
    • Cullin-5 antibody
    • Cullin5 antibody
    • VACM 1 antibody
    • VACM-1 antibody
    • VACM1 antibody
    • Vasopressin activated calcium mobilizing receptor 1 antibody
    • Vasopressin activated calcium mobilizing receptor antibody
    • Vasopressin-activated calcium-mobilizing receptor 1 antibody
    • Vasopressin-activated calcium-mobilizing receptor-1 antibody
    see all

Anti-Cullin 5 antibody - Carboxyterminal end images

References for Anti-Cullin 5 antibody - Carboxyterminal end (ab82292)

This product has been referenced in:
  • Carlisle AJ  et al. CXCR4 expression heterogeneity in neuroblastoma cells due to ligand-independent regulation. Mol Cancer 8:126 (2009). WB . Read more (PubMed: 20028517) »

See 1 Publication for this product

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I am sorry but we dont' have specific information available regarding the appearence of smaller than expected band size for CUL5.Thiswestern may show the existance of a cell type-specificisoform present in HeLa c...

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