Overview

  • Product name
  • Description
    Rabbit polyclonal to Cullin 7
  • Specificity
    Ab34841 is recognizes Cullin 7, but cross reactivity with other human cullins may occur.
  • Tested applications
    Suitable for: IP, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    RSRGPVYASCTATQSFSTFR

    conjugated to KLH, corresponding to C terminal amino acids 1679 - 1698 of Human Cullin 7.

  • Positive control
    • IP: Protein from cell lysates (using HeLa, NIH-3T3, and others). Most cell lines expressing Cullin 7 can be used as a positive control.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: Whole serum
  • Concentration information loading...
  • Purification notes
    Ab34841 is a monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
ELISA
WB
  • Application notes
    ELISA: 1/2000 - 1/10000.
    IP: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of a probable SCF-like E3 ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably plays a role in the degradation of proteins involved in endothelial proliferation and/or differentiation (By similarity). Seems not to promote polyubiquitination and proteasomal degradation of TP53. In vitro, complexes of CUL7 with either CUL9 or FBXW8 or TP53 contain E3 ubiquitin-protein ligase activity.
    • Tissue specificity
      Highly expressed in fetal kidney and adult skeletal muscle. Also abundant in fetal brain, as well as in adult pancreas, kidney, placenta and heart. Detected in trophoblasts, lymphoblasts, osteoblasts, chondrocytes and skin fibroblasts.
    • Pathway
      Protein modification; protein ubiquitination.
    • Involvement in disease
      Defects in CUL7 are the cause of 3M syndrome type 1 (3M1) [MIM:273750]. An autosomal recessive disorder characterized by severe pre- and postnatal growth retardation, facial dysmorphism, large head circumference, and normal intelligence and endocrine function. Skeletal changes include long slender tubular bones and tall vertebral bodies.
    • Sequence similarities
      Belongs to the cullin family.
      Contains 1 DOC domain.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CUL-7 antibody
      • CUL7 antibody
      • CUL7_HUMAN antibody
      • Cullin-7 antibody
      • dJ20C7.5 antibody
      • KIAA0076 antibody
      see all

    References

    ab34841 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Jurkat cells)
    Loading amount
    100000 cells
    Specification
    Jurkat cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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