Overview

  • Product nameAnti-CX3CL1 antibody
    See all CX3CL1 primary antibodies
  • Description
    Rabbit polyclonal to CX3CL1
  • Tested applicationsSuitable for: Blocking, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hFractalkine (human Fractalkine)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent dilution.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use a concentration of 0.5 µg/ml.
WB Use a concentration of 0.1 - 0.2 µg/ml.

Target

  • FunctionThe soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.
  • Tissue specificitySmall intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the intercrine delta family.
  • Post-translational
    modifications
    A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A 152E5.2 antibody
    • AB030188 antibody
    • ABCD 3 antibody
    • ABCD3 antibody
    • AI848747 antibody
    • C-X3-C motif chemokine 1 antibody
    • C3Xkine antibody
    • Chemokine (C-X3-C motif) ligand 1 antibody
    • Chemokine C X3 C motif ligand 1 antibody
    • Chemokine CX3C Motif Ligand 1 antibody
    • CX3C membrane anchored chemokine antibody
    • CX3C membrane-anchored chemokine antibody
    • Cx3cl1 antibody
    • CXC 3 antibody
    • CXC3 antibody
    • CXC3C antibody
    • D8Bwg0439e antibody
    • FKN antibody
    • Fractalkine antibody
    • Neurotactin antibody
    • NTN antibody
    • NTT antibody
    • Processed fractalkine antibody
    • SCYD 1 antibody
    • SCYD1 antibody
    • Small inducible cytokine D1 antibody
    • Small inducible cytokine subfamily D (Cys X3 Cys) member 1 antibody
    • small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin) antibody
    • Small inducible cytokine subfamily D member 1 antibody
    • Small-inducible cytokine D1 antibody
    • X3CL1_HUMAN antibody
    see all

Anti-CX3CL1 antibody images

  • IHC image of CX3CL1 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9819, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-CX3CL1 antibody (ab9819)

This product has been referenced in:
  • Barlic J  et al. Atherogenic lipids induce adhesion of human coronary artery smooth muscle cells to macrophages by up-regulating chemokine CX3CL1 on smooth muscle cells in a TNFalpha-NFkappaB-dependent manner. J Biol Chem 282:19167-76 (2007). Read more (PubMed: 17456471) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. ab9819 will recognize the chemokine domain of human Fractalkine (76 a.a.). The chemokine domain is amino acids 25-100 of the extracellular domain (25-341). The sequence of which is as follows: QHHGVTKCNI TCSKMTSKIP V...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"