• Product nameAnti-CX3CR1 antibody
    See all CX3CR1 primary antibodies
  • Description
    Rabbit polyclonal to CX3CR1
  • Tested applicationsSuitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CX3CR1.

    (Peptide available as ab64633.)

  • Positive control
    • This antibody gave a positive signal in the following Human Lysates: HeLa Whole Cell, SW480 Whole Cell, Human Small Intestine Tissue, JEG-3 Whole Cell, Y79 Whole Cell, HepG2 Whole Cell, Caco 2 Whole Cell


Associated products


Our Abpromise guarantee covers the use of ab51668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40,50 kDa (predicted molecular weight: 40 kDa).Can be blocked with CX3CR1 peptide (ab8125).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionReceptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
  • Tissue specificityExpressed in lymphoid and neural tissues.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta chemokine receptor-like 1 antibody
    • C X3 C CKR 1 antibody
    • C-X3-C CKR-1 antibody
    • CCRL1 antibody
    • Chemokine C X3 C motif receptor 1 antibody
    • CMK BRL 1 antibody
    • CMK-BRL-1 antibody
    • CMK-BRL1 antibody
    • CMKBLR1 antibody
    • CMKDR1 antibody
    • CX3C chemokine receptor 1 antibody
    • CX3C CKR1 antibody
    • CX3C1_HUMAN antibody
    • CX3CR1 antibody
    • Fractalkine receptor antibody
    • G-protein coupled receptor 13 antibody
    • GPR13 antibody
    • GPRV28 antibody
    • V28 antibody
    see all

Anti-CX3CR1 antibody images

  • CX3CR1 was immunoprecipitated using 0.5mg JEG3 whole cell extract, 5µg of Rabbit polyclonal to CX3CR1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, JEG3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab51668.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 40kDa; CX3CR1

  • All lanes : Anti-CX3CR1 antibody (ab51668) at 1 µg

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 whole cell lysate (ab3957)
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 5 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : Caco 2 whole cell lysate (ab3950)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40,50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 130 kDa. We are unsure as to the identity of these extra bands.
    CX3CR1 has a predicted MW of 40 kDa and has 3 known isoforms (SwissProt). The 50 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to CX3CR1.
  • ICC/IF image of ab51668 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51668, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CX3CR1 antibody (ab51668)

ab51668 has not yet been referenced specifically in any publications.

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I am very pleased to hear you would like to accept our offer and test ab51668 in rhesus monkey. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rhesus monkey and include t...

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Thank you for your inquiry.
The immunogen sequence is N-terminal and shares 90% sequence homology with Rhesus macaque.
I hope this information helps. Please contact us with any other questions.

Thank you for your enquiry and your interest in our products. All of these 4 antibodies (ab8021, ab7200, ab7201, ab51668) recognize CX3CR1 and are suitable for Western blot application. However, ab7200 has been tested and characterized for detecting ra...

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