Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CX3CR1.
(Peptide available as ab64633.)
Our Abpromise guarantee covers the use of ab51668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40,50 kDa (predicted molecular weight: 40 kDa).Can be blocked with CX3CR1 peptide (ab8125).|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
CX3CR1 was immunoprecipitated using 0.5mg JEG3 whole cell extract, 5µg of Rabbit polyclonal to CX3CR1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, JEG3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab51668.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 40kDa; CX3CR1
ab51668 has not yet been referenced specifically in any publications.