Overview

  • Product name
  • Description
    Rabbit polyclonal to CX3CR1
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to amino acids 175-189 of Human CX3CR 1.

  • Positive control
    • THP-1 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab8020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
WB 1/500. Predicted molecular weight: 50 kDa.Can be blocked with CX3CR1 peptide (ab8124).

Target

  • Function
    Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
  • Tissue specificity
    Expressed in lymphoid and neural tissues.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta chemokine receptor-like 1 antibody
    • C X3 C CKR 1 antibody
    • C-X3-C CKR-1 antibody
    • CCRL1 antibody
    • Chemokine C X3 C motif receptor 1 antibody
    • CMK BRL 1 antibody
    • CMK-BRL-1 antibody
    • CMK-BRL1 antibody
    • CMKBLR1 antibody
    • CMKDR1 antibody
    • CX3C chemokine receptor 1 antibody
    • CX3C CKR1 antibody
    • CX3C1_HUMAN antibody
    • CX3CR1 antibody
    • Fractalkine receptor antibody
    • G-protein coupled receptor 13 antibody
    • GPR13 antibody
    • GPRV28 antibody
    • V28 antibody
    see all

Anti-CX3CR1 antibody images

  • All lanes : Anti-CX3CR1 antibody (ab8020) at 1/500 dilution

    Lane 1 : THP-1 cell lysate with absence of blocking peptide
    Lane 2 : THP-1 cell lysate with presence of blocking peptide


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ab8020 at 10µg/ml staining CX3CR1 in human spleen cells by IHC

  • Immunofluorescence of CX3CR1 in THP1 cells using ab8020 at 20 ug/ml.

References for Anti-CX3CR1 antibody (ab8020)

This product has been referenced in:
  • Ertürk A  et al. Interfering with the Chronic Immune Response Rescues Chronic Degeneration After Traumatic Brain Injury. J Neurosci 36:9962-75 (2016). IHC-FrFl ; Mouse . Read more (PubMed: 27656033) »
  • Szukiewicz D  et al. CX3CL1 (fractalkine) and TNFa production by perfused human placental lobules under normoxic and hypoxic conditions in vitro: the importance of CX3CR1 signaling. Inflamm Res 63:179-89 (2014). IHC-P ; Human . Read more (PubMed: 24270813) »

See all 5 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bone Marrow-Derived Macrophages)
Permeabilization
Yes - 0.1% TritonX-100
Specification
Bone Marrow-Derived Macrophages
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 10 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain tissue)
Permeabilization
Yes - 0.5% triton
Specification
brain tissue
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formaldehyde
Username

Dr. Ali Ertürk

Verified customer

Submitted Oct 24 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cell (Brain slice - CA1 hippocampal section)
Permeabilization
Yes - with blocking
Specification
Brain slice - CA1 hippocampal section
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 13 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Adult mouse brain. LANE NUMBER 2 IN THE FIGURE.)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
80 µg
Specification
Adult mouse brain. LANE NUMBER 2 IN THE FIGURE.
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 02 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tooth)
Antigen retrieval step
None
Permeabilization
No
Specification
tooth
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 30 2015

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (gel 10%)
Sample
Mouse Cell lysate - whole cell (Epithelial ovarian cancer cell line)
Specification
Epithelial ovarian cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Hilal Gurler

Verified customer

Submitted Jun 18 2013

Thank you for your enquiry. As far as we are aware, cross reactivity with mouse has not yet been tested for use with ab7201. Also, ab8020 has not yet been tested for application in IHC-frozen sections (the antibody has only so far been tested in Wester...

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